Abstract: In this paper, a steganalytical scheme based on image difference histogram analysis is proposed on the basis of the effect on image difference histogram caused by the LSB steganography scheme against statistical analysis proposed by Zhang Xin-peng et al. The scheme uses the histogram transfer matrix between the cover image and stego image, combining fitness bias caused by using generalized Laplacian distribution to fit the difference histogram, to estimate the embedded message length. Experiments show that our method has preferable accuracy.